Product | Catalog No. | Specification | Shelf life | Price |
---|---|---|---|---|
BETAGENE rhIL-12 | GE11.1 | 10μg | 24 months | 1400 |
GE11.2 | 50μg | 3800 |
Product | CAT. No. | Volumn | Shelf life | Price |
---|---|---|---|---|
rhIL-12 | GE11.1 | 10μg | 24 months | 1400 |
GE11.2 | 50μg | 3800 |
IL-12 is a heterodimeric cytokine of disulfide-linked p35 and p40 subunits. It is secreted by monocytes, macrophages, dendritic cells, neutrophils, Langerhans cells, keratinocytes, microglia, and peripheral B cells. In humans, the p40 subunit can also associate with a p19 subunit to form IL-23. IL-12 signals through a receptor complex consisting of IL-12 R beta 1 and IL-12 R beta 2. It promotes Th1 immune responses by inducing IFN-gamma secretion from NK cells, T cells, and macrophages and by enhancing NK cell and T cell mediated cytotoxicity. It also cooperates with IL-18 in the generation of Th1 memory cells.
储存分类代码 | WGK | 闪点(F) |
13 - Non Combustible Solids | WGK 3 | Not Applicable |
闪点(C) | 个人防护装备 | |
Not Applicable | dust mask type N95 (US), Eyeshields, Gloves |
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder
Gene ID
Catalog Number
GE11.1/GE11.2
Unit Size
10μg/50μg
Source
E. coli
Molecular Weight
Approximately 75kDa, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit.
Purity (HPLC)
>95%
Purity (SDS-PAGE)
>95%
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated human T lymphoblasts is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg.
Host Cell DNA Residue
<0.02 ng/μg of protein tested by DNA Fluorescent Staining method
Host Cell Protein Residue
<0.5 ng/μg of protein tested by ELISA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.
Sterility
Negative
Endotoxin
<0.01 EU/μg as determined by LAL gel-clot method
Mycoplasma
Negative
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended in our USER MANUAL
Reconstitution Stability & Storage
Please read our USER MANUAL and FAQs
Specs
DATA
FAQs
Sterile Filtered White lyophilized (freeze-dried) powder.
IL-12 is a heterodimeric cytokine of disulfide-linked p35 and p40 subunits. It is secreted by monocytes, macrophages, dendritic cells, neutrophils, Langerhans cells, keratinocytes, microglia, and peripheral B cells. In humans, the p40 subunit can also associate with a p19 subunit to form IL-23. IL-12 signals through a receptor complex consisting of IL-12 R beta 1 and IL-12 R beta 2. It promotes Th1 immune responses by inducing IFN-gamma secretion from NK cells, T cells, and macrophages and by enhancing NK cell and T cell mediated cytotoxicity. It also cooperates with IL-18 in the generation of Th1 memory cells.
GE11.1/GE11.2
10μg/50μg
CHO
Approximately 75kDa, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit.
p35 Subunit: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLA
SRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVM
SYLNAS
p40 Subunit: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIW
STDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMV
DAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRY
YSSSWSEWASVPCS
>95%
>98%
The ED50 as determined by a cell proliferation assay using PHA-activated human T lymphoblasts is <0.05 ng/mL, corresponding to a specific activity of > 2.0 × 10^7 Units/mg.
<0.02 ng/μg of protein tested by DNA Fluorescent Staining method.
<0.5 ng/μg of protein tested by ELISA.
Lyophilized from a 0.2μm filtered solution in PBS pH7.4
Negative
<0.01 EU/μg as determined by LAL gel-clot method
Negative
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended in our USER MANUAL
Please read our USER MANUAL and FAQs.