汇融/贝融生物 - 专业的蛋白制剂提供商

E.Coli Recombinant

BETAGENE Human Interleukin-21

Product	Catalog No.	Specification	Shelf life	Price
BETAGENE rhIL-21	GE08.1	50μg	24 months	3100
BETAGENE rhIL-21	GE08.2	100μg	24 months	5000

Product	CAT. No.	Volumn	Shelf life	Price
rhIL-12	GE11.1	10μg	24 months	1400
rhIL-12	GE11.2	50μg	24 months	3800

产品描述

158997890645513199695db917eed1868815883f56b63

IL-12 is a heterodimeric cytokine of disulfide-linked p35 and p40 subunits. It is secreted by monocytes, macrophages, dendritic cells, neutrophils, Langerhans cells, keratinocytes, microglia, and peripheral B cells. In humans, the p40 subunit can also associate with a p19 subunit to form IL-23. IL-12 signals through a receptor complex consisting of IL-12 R beta 1 and IL-12 R beta 2. It promotes Th1 immune responses by inducing IFN-gamma secretion from NK cells, T cells, and macrophages and by enhancing NK cell and T cell mediated cytotoxicity. It also cooperates with IL-18 in the generation of Th1 memory cells.

安全数据

储存分类代码	WGK	闪点（F）
13 - Non Combustible Solids	WGK 3	Not Applicable
闪点（C)	个人防护装备
Not Applicable	dust mask type N95 (US), Eyeshields, Gloves

产品信息

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder

Gene ID

3592

Catalog Number

GE11.1/GE11.2

Unit Size

10μg/50μg

Source

E. coli

Molecular Weight

Approximately 75kDa, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit.

Purity (HPLC)

＞95%

Purity (SDS-PAGE)

＞95%

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using PHA-activated human T lymphoblasts is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.

Host Cell DNA Residue

＜0.02 ng/μg of protein tested by DNA Fluorescent Staining method

Host Cell Protein Residue

＜0.5 ng/μg of protein tested by ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.

Sterility

Negative

Endotoxin

＜0.01 EU/μg as determined by LAL gel-clot method

Mycoplasma

Negative

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended in our USER MANUAL

Reconstitution Stability & Storage

Please read our USER MANUAL and FAQs

Specs
DATA
FAQs

Physical Appearance

Gene ID

Description

Catalog Number

Unit Size

Source

Molecular Weight

AA Sequence

Purity (HPLC)

Purity (SDS-PAGE)

Biological Activity

Host Cell DNA Residue

Host Cell Protein Residue

Formulation

Sterility

Endotoxin

Mycoplasma

Shipping

Reconstitution Stability & Storage

Sterile Filtered White lyophilized (freeze-dried) powder

Human IL-21 is encoded by IL-21 gene located on Chr. 4. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies.

GE08.1/GE08.2

50μg/100μg

E. coli

Approximately 15.4kDa, a single non-glycosylated polypeptide chain containing 133 amino acid QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYE

KKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS

＞97%

Assay 1: Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 is ＜2 ng/mL, corresponding to a specific activity of ≥ 5.0 × 10^5 Units/mg. Assay 2: The ED50 as determined by a cell proliferation assay using human N1186 T cells is ＜20 ng/mL, corresponding to a specific activity of ≥ 5.0 × 10^4 Units/mg.

＜0.02 ng/μg of protein tested by DNA Fluorescent Staining method.

＜0.5 ng/μg of protein tested by ELISA.

Lyophilized from a 0.2μm filtered solution in PBS pH7.4.

Negative

＜0.01 EU/μg as determined by LAL gel-clot method.

Negative

59067

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended in our USER MANUAL

Please read our USER MANUAL and FAQs