Sterile Filtered White lyophilized (freeze-dried) powder

7448

Vitronectin (VTN or VN) is a glycoprotein of the hemopexin family which is abundantly found in serum, the extracellular matrix and bone. In humans it is encoded by the VTN gene. Vitronectin binds to integrin αVβ3 and thus promotes cell adhesion and spreading. It also inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to several serpins (serine protease inhibitors). It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.

GB13.1

300μg

HEK293

VTN consists of 468 amino acids and predicts a molecular weight of 53.4 kDa. As a result of glycosylation, it migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.

A DNA sequence encoding the human VTN (Met1-Leu478) was expressed with a polyhistidine tag at the C-terminus.

＞95%

＞95%

Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to Vitronectin-coated plates (10 μg/mL and 100 μL/well), approximately ＞40% cells will adhere specifically after 30 minutes at 37 ℃.

Lyophilized from sterile PBS, pH 7.4.

Negative

＜0.01 EU/μg as determined by LAL gel-clot method

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